제품 상세정보

Anti-Psoriasis Drug Screening

The Model

The Psoriasis tissue model is cultured using normal human epidermal keratinocytes and psoriatic fibroblasts harvested from psoriatic skin tissue specimens. The cells are cultured on specially prepared tissue culture inserts using serum free medium to form a multilayered, highly differentiated tissue. The reconstructed psoriasis tissues adopt a psoriatic phenotype as evidenced by increased basal cell proliferation, expression of psoriasis-assosiated biomarkers, and elevated cytokine release. The model provides researchers with a useful, in vitro means to assess the pre-clinical safety and efficacy of lead therapeutic compounds. For more information on the Psoriasis model.

The Method

Evaluation of Psoriasis Drug Formulations Using a Human Tissue Model of Psoriasis

Effect of calipotriol on psoriatic biomarkers - Repeat exposure (once daily) for 4 days 

Calcipotriol treatment: IL-6 release from Psoriasis tissue model

Effects of IL-17 on Chemokines and Cytokines that Mediate Chronic Inflammation in Psoriasis

As increasing evidence implicates the TH17 pathway as playing an important role in the pathophysiology of Psoriasis, the effects of IL-17 were investigated on MatTek's Psoriasis tissue model in a tightly controled in vitro setting.  Exogenous IL-17 induced the secretion of chemokines and cytokines that mediate chronic inflammation in Psoriasis including CCL20 (MIP-3α, Th17 and Dendritic Cell chemoattractant), IL-8 (Neutrophil chemoattractant) and IL-6 (Th17 Cell survival).  These results demonstrate that IL-17 exacerbates inflammation in an in vitro human cell-based model of Psoriasis.

Effect of IL-17 on Psoriatic Biomarkers 

Technical References

702. DEVELOPMENT AND CHARACTERIZATION OF 3D PSORIATIC TISSUE MODEL.

Ayehunie¹, S., Hedin¹, C., Landry¹, T., Cataldo¹, A., Spratt¹, M., Clark², R., Kupper², T. and Klausner¹, M. ¹MatTek Corporation, Ashland, MA, ²Brigham and Women’s Hospital, Boston, MA. Presented at Society of Investigative Dermatology Meeting 2012...

701. DELPHINIDIN INDUCES EPIDERMAL DIFFERENTIATION AND INHIBITS PROLIFERATION AND INFLAMMATION IN A THREE-DIMENSIONAL RECONSTITUTED SKIN MODEL OF PSORIASIS.

Pal¹, H.C., Sharma¹, S., Ayehunie², S. and Afaq¹, F. ¹Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA ²MatTek Corporation, 200 Homer Avenue, Ashland, MA, USA Presented at Society of Investigative Dermatology Meeting 2012...